BACKGROUND: The purpose of this study was to identify the main greenhouse gas (GHG) emitting activities or products among the medical devices (MD) and medicines used in a polyvalent Intensive Care Unit (ICU). METHODS: A pragmatic eco-audit was conducted in a 21-beds polyvalent ICU, in Saint-Brieuc, Bretagne, France. It consisted of estimating GHG emissions of products or activities, considering process-based life cycle analysis (LCA), economic input-output analysis (EIO) and hybrid-LCA. Results were expressed as Carbon Dioxide Equivalent (CO RESULTS: With remaining uncertainty, GHG emissions were estimated at 61.1 kgCO CONCLUSION: Upcoming studies focusing on actions on these particular hot spots would be of interest in order to significantly decrease GHG emissions but also to increase resilience of critical care.