UNLABELLED: Non-synonymous single nucleotide polymorphisms (nsSNPs), also known as missense SNPs, can seriously affect an individual's vulnerability to numerous diseases, including cancer. In this study, we conducted a comprehensive in-silico analysis to examine the structural and functional implications of nsSNPs within the Folate Hydrolase 1( SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40203-025-00319-3.