Contrast-enhanced optical microscopy using a planar photonic substrate and dark-field illumination.

• Tác giả: Chunzheng Bai, Yurong Cao, Mengping Qi, Dong Wang, Yong-Hong Ye
• Ngôn ngữ: eng
• Ký hiệu phân loại: 616.047 Symptoms and general pathological processes as problems in their own right
• Thông tin xuất bản: United States : Optics letters , 2025
• Bộ sưu tập: NCBI
• ID: 728410

Tóm tắt

Visualization of low-index dielectric nanoparticles and biological nanostructures is challenging under a traditional optical microscope. In this work, we propose a planar photonic substrate to enhance the extremely weak scattering signal and improve the contrast in imaging low-index samples under a dark-field illumination (DFI) optical microscope. Specifically, a planar photonic substrate is prepared by depositing a multilayer optical film on a silica substrate with the maximum electric field intensity distributed on the substrate surface. The scattering signal of a sample placed on the planar photonic substrate is enhanced due to the strong substrate-nanoparticle interaction. The experimental results show that the scattering intensity of a 150-nm-diameter SiO