BACKGROUND: A systematic global map on toxigenesis and genomic virulence of Vibrio spp. was analyzed for research progress to identify the emerging research patterns for establishing a database for designing future interventions. METHOD: The databases (Web of Science and Scopus) were analyzed with Voxviewer software to map the global scale of Vibrio spp. or virulence toxin/genes publications and standardized using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) strategies. RESULTS: The results identified 1162 (Web of Science n = 620, Scopus n = 542), while 314 studies qualified for inclusion and were significantly analyzed on virulence toxin/genes. By co-citation analysis, 4-thematic clusters were developed from 6420 citations and a total reference of 9062. Cluster #1 (pathogenesis & virulence factors) and cluster #4 (host response factors) generated the utmost publications and citations (n = 40, 643) and the least (n = 7, 85) interest by the researcher. Whereas 8-thematic clusters were developed by bibliographic coupling technique analysis, cluster#1 and cluster#8 generated the utmost (n = 78, 1684) and least (n = 7, 81) publications and citations interest by the researcher. CONCLUSIONS: Our findings suggest that Vibrio toxigenesis and virulence factors are a complex field requiring an interdisciplinary approach consisting of interconnected perspectives that have important consequences for academics and policymakers.