The mysterious case of missing lymphocytes: a cautionary tale of interinstitutional variability in outcomes of lung dissociation protocols.

• Tác giả: Elise Armstrong, Olivia S Harlow, Fang Ke, Joseph P Mizgerd, Vijay Raaj Ravi, Nathan L Sanders, Anukul T Shenoy
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : American journal of physiology. Lung cellular and molecular physiology , 2025
• Bộ sưu tập: NCBI
• ID: 730244

Tóm tắt

Rigor and reproducibility are vital to scientific advancement. It is unclear whether a protocol optimized for tissue dissociation in one institution performs well universally. Here, we share our brand-new lab's experience with interinstitutional variability that led to the discovery that a protocol optimized for murine lung dissociation at Boston University (BU) fails to reproduce similar CD4