Alkaline stress impairs fish productivity and performance and, therefore, is considered one of the major challenges facing aquaculture. In this work, the effects of supplementing diets with camel whey protein hydrolysates (WPH) on growth, digestion, antioxidant capacity, and gene expression were investigated in Nile tilapia (Oreochromis niloticus) under alkaline stress. A total of 160 fish (16.17 ± 0.29 g) were equally assigned into four treatments, with 10 fish in each replicate. The control (C) and WPH groups received the basal diet supplemented with 0 and 75 g/kg WPH, respectively, and were reared in freshwater with an alkalinity of 1.4 mmol NaHCO