The human health and ecotoxicity impacts of agricultural pesticide use in Iran in 2022 were estimated. The impacts of agricultural pesticide use in Iran by pesticide, crop, and province were assessed based on the USEtox model in terms of disability-adjusted life year (DALY) for human health and potentially disappeared fraction of freshwater ecosystem species (PDF) for ecotoxicity. The annual mass of agricultural pesticide use in Iran in 2022 was 17,188 tons, consisting of herbicides (46.2 %), insecticides (30.0 %), and fungicides (23.8 %). The DALYs and DALY rate (per 100,000 people) of agricultural pesticide use in Iran were determined to be 25,140 and 29.4, respectively. The ecotoxicity impact of agricultural pesticide use in Iran was calculated to be 3.35 × 10