Flupyrimin is an emerging neonicotinoid insecticide primarily used to control rice planthoppers. However, knowledge gaps exist regarding its uptake and transport in rice planting systems. Elucidating the absorption and distribution properties of flupyrimin in rice will help assess the potential risks of human exposure to flupyrimin via the food chain. Here, we studied the uptake kinetics and transport mechanisms of flupyrimin in rice plants grown under hydroponic and soil conditions. The hydroponic experiment indicated that flupyrimin was easily taken up by rice roots via a symplastic passive diffusion process and was mainly distributed in the cell soluble fractions (50.6 %－88.0 %). Compared with transportation from the roots to the stems, flupyrimin was ultimately transported from the stems to the leaves with a greater translocation factor (TF) (TF