Low-cadmium (Cd)-accumulating rice (Oryza sativa L.) cultivated by OsNRAMP5 mutation is a promising approach for ensuring food safety and human health. However, the impacts of reduced manganese (Mn) uptake caused by OsNRAMP5 mutation on rice growth and Cd accumulation under varying Mn levels remains unknown. Here, hydroponics and pot experiments were used to investigate the growth and Cd accumulation of an OsNRAMP5 mutant rice, Zhong'an 7 (ZA7), and the wild type, Zhongzao 35 (ZZ35), under different Mn supply conditions, and the Mn thresholds for ZA7 were determined. There was lower biomass of ZA7 than ZZ35 under hydroponic conditions with 0-0.05 μM Mn supply, and this was alleviated at Mn concentrations ranging from 1 to 200 μM. In pot Experiment I the grain yields of ZA7 in five soils increased from 5.92 to 15.0 g pot