Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy.

• Tác giả: Xiong Chen, Patrick Eiring, Johan Hofkens, Volker Leen, Markus Sauer, Gang Wen
• Ngôn ngữ: eng
• Ký hiệu phân loại: 005.114 +*Functional programming
• Thông tin xuất bản: United States : Journal of the American Chemical Society , 2025
• Bộ sưu tập: NCBI
• ID: 731807
• Chủ đề: 1. Functionalized; 2. Docetaxel; 3. Probes

Tóm tắt

Visualizing the ultrastructure of mitotic spindles, the macromolecular machines that segregate chromosomes during mitosis, by fluorescence imaging remains challenging. Here we introduce an azido- and amino-functionalized docetaxel probe, which upon labeling of microtubules can be fixed, click-labeled and linked into hydrogels. The new probe is particularly useful for super-resolution imaging of dense microtubule structures in mitotic spindles. Multicolor expansion microscopy of mitotic cells allowed us to visualize the different phases of mitosis with unprecedented spatial resolution.