A high-throughput protein tagging toolkit that retains endogenous untranslated regions for studying gene regulation in kinetoplastids.

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Tác giả: Carla Gilabert Carbajo, Xiaoyang Han, Bhairavi Savur, Fatima Taha, Calvin Tiengwe, Michele Tinti, Arushi Upadhyaya, Richard J Wheeler, Phillip A Yates

Ngôn ngữ: eng

Ký hiệu phân loại: 296.431 High Holy Days

Thông tin xuất bản: England : Open biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 732736

Kinetoplastid parasites cause diseases that threaten human and animal health. To survive transitions between vertebrate hosts and insect vectors, these parasites rely on precise regulation of gene expression to adapt to environmental changes. Since gene regulation in kinetoplastids is primarily post-transcriptional, developing efficient genetic tools for modifying genes at their endogenous loci while preserving regulatory mRNA elements is crucial for studying their complex biology. We present a CRISPR/Cas9-based tagging system that preserves untranslated regulatory elements and uses a viral 2A peptide from
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