BACKGROUND: Given the inherent limitations of invasive biopsy and the insufficient accuracy of liver-related serum biomarkers, there is an urgent need for the development of reliable, non-invasive imaging techniques for the diagnosis of liver fibrosis. This study aims to investigate the correlation between magnetic resonance imaging (MRI) T1/T2 mapping sequences and biomarkers of collagen deposition and ongoing systemic inflammation, and to evaluate the potential of T1/T2 mapping as a non-invasive method for the accurate diagnosis of liver fibrosis. METHODS: A mouse model of carbon tetrachloride (CCl RESULTS: The principal findings indicated that T1 and T2 values exhibited a progressive increase with the severity of fibrosis, demonstrating a positive correlation with collagen deposition and inflammatory factors, especially the hydroxyproline content (r = 0.880, P <
0.002). The HYP content exhibited a progressive increase with advancing fibrosis stages (ρ = 0.914, P <
0.002). Similarly, T1 values increased significantly across fibrosis stage(ρ = 0.854, P <
0.002). Statistical comparison of these coefficients revealed no significant difference (Z = 1.031, P = 0.303). ROC curve analysis showed that T1 mapping was more accurate than T2 mapping in detecting collagen deposition and inflammation. CONCLUSIONS: This study highlighted the potential of T1/T2 mapping as non-invasive and quantitative biomarkers for diagnosing and staging liver fibrosis, providing new insights into the onset and progression of liver fibrosis.