OBJECTIVE: The objective of this study was to evaluate the effectiveness and safety of endoscopic mucosal resection assisted by a transparent cap (EMR-C) for rectal neuroendocrine tumor (R-NET) by novice endoscopists of digestive endoscopy center. METHODS: R-NET patients consulted in department of digestive endoscopy center of Guangdong Provincial Peolple's Hospital from 2022 to 2023, were recruited in this study. Novice endoscopists who had no ESD experience before were randomly to accomplish the EMR-C operation. Each novice endoscopist separately completed at least 3 cases after simple training by teachers and the operation time was counted on average. Vertical and horizontal margins of the specimens were further analyzed to determine whether radical resection was successful or not. R-NET patients were followed up at 12 months after operation. RESULTS: 30 cases of R-NET patients (13 males and 17 females, (53.83 ± 13.31) y), originated from the deep or submucosal layers of the intestinal wall mucosa by ultrasound colonoscopy, were recruited in all of our study. 10 novice endoscopists (each for 3 cases) were arranged to complete EMR-C operations by 2 teachers. The average time was (8.87 ± 1.87) minutes and the sections were clean and no bleeding and further clipped by 2-4 metal clips. Pathological and immunohistochemical results were further made to confirm the diagnosis. The en bloc resection rate was 100.00% and the R0 rate was 83.33% (horizontal margins were 100.00% negative and the vertical margins were 83.33% negative). No recurrence was found during the followed up at 12 months after operation. CONCLUSIONS: EMR-C is safe and efficient for R-NET, and easily manageable for novice endoscopists of digestive endoscopy center.