Ice-bath-effervescence-assisted salting-out extraction combined with microwave-assisted derivatization for the detection of spermidine and spermine in whole blood.

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Tác giả: Wen-Rong Chen, Chia-Hsien Feng, Fang-Yi Liao, Chi-Yu Lu, Jing-Ru Weng

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Netherlands : Journal of chromatography. A , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 733641

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Spermidine (Spd) and spermine (Spm) are biomarkers for various cancers. In the present study, we used 4-dimethylamino-1-naphthyl isothiocyanate as a derivatizing reagent to achieve microwave-assisted nucleophilic addition to Spd and Spm. We designed a strategy comprising an ice bath combined with salting out to increase the efficiency of Spd and Spm derivative extractions. Ice-bath-effervescence-assisted salting-out extraction (IEA-SOE) was conducted using a proton donor (citric acid) and an effervescent salt (KHCO

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