The current study aimed to isolate Beauveria brongniartii conidia from forest soils, identify the fungus, and evaluate its effectiveness on the eggs, larvae, pupae, and adults of Spodoptera litura. Insect mortality rates were recorded every 3, 6, 9, and 12 days. The identification of entomopathogenic fungi was carried out using molecular techniques, including PCR, DNA sequencing, and molecular markers, to detect species-specific 18 S rDNA genetic sequences, all performed under aseptic conditions. The results indicated that higher conidia concentrations (2.7 × 10