PRODE recovers essential and context-essential genes through neighborhood-informed scores.

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Tác giả: Riccardo Bevilacqua, Thomas Cantore, Yari Ciani, Francesca Demichelis, Paola Gasperini, Eytan Ruppin, Sanju Sinha

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Genome biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 734524

Gene context-essentiality assessment supports precision oncology opportunities. The variability of gene effects inference from loss-of-function screenings across models and technologies limits identifying robust hits. We propose a computational framework named PRODE that integrates gene effects with protein-protein interactions to generate neighborhood-informed essential (NIE) and neighborhood-informed context essential (NICE) scores. It outperforms the canonical gene effect approach in recovering missed essential genes in shRNA screens and prioritizing context-essential hits from CRISPR-KO screens, as supported by in vitro validations. Applied to Her2 + breast cancer tumor samples, PRODE identifies oxidative phosphorylation genes as vulnerabilities with prognostic value, highlighting new therapeutic opportunities.
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