BACKGROUND: Fluid dynamics during and after a septic event is complex, but better knowledge could guide both fluid resuscitation and fluid removal. We aimed to compare fluid dynamics before and after sepsis in a clinically relevant mono-bacterial porcine model. METHODS: Twelve sows with a mean body weight of 56 kg were anesthetized, mechanically ventilated, and invasively monitored. Sepsis was induced with an intravenous infusion of P. aeruginosa. Animals were resuscitated during the acute septic phase according to a protocolized algorithm. Volume kinetics was studied before the bacterial infusion (baseline) and 24 h later (late sepsis), and both consisted of an infusion of 1,500 mL of 0.9% saline over 20 min with repeated hemoglobin and albumin measurements and urine quantification. RESULTS: The kinetic analysis at baseline showed transient volume expansion of the central fluid compartment (the plasma) and a fast-exchange interstitial space, while gradually more fluid accumulated in the remote "third fluid space" with very slow turnover. In the late sepsis phase, hypoalbuminemia and slight hypovolemia was observed. As compared with baseline, fluid kinetics showed improved plasma expansion, and more expansion of the fast-exchange interstitial space rather than the slow-exchange space. The rate constant k CONCLUSION: After sepsis, fluid was accumulated in the slow-exchange compartment, and further fluid administration distributed preferentially to the fast-exchange compartment with acceleration of lymph flow, improved plasma expansion, and recruitment of interstitial albumin.