BACKGROUND: The gut-brain axis plays a crucial role in mitigating the adverse effects of environmental agents such as nickel exposure. Nickel, recognized as a heavy metal, poses significant concerns for public health because of its impact on neurological disorders and oxidative stress
consequently, it is prioritized for evaluations of its effects on biological pathways. This study investigates the potential of native probiotic strains to modulate inflammatory and autophagy signaling pathways, which are vital for combating oxidative stress. METHODS: Twenty male NMRI mice were divided into 4 groups randomly and were gavaged with NiCl RESULTS: Our findings demonstrated that treatment with this cocktail of native probiotic Lactobacillus spp. and Bifidobacterium spp. significantly increased the expression of autophagy genes compared to the control group exposed to NiCl CONCLUSION: The beneficial effects of our native probiotic strains were confirmed through enhanced expression of autophagy genes and reduced neuroinflammation, suggesting their potential as therapeutic agents in mitigating the adverse impacts of nickel exposure on brain health via modulation of the gut-brain axis.