Laboratory assessment of recovery of porcine circovirus 2 and porcine reproductive and respiratory syndrome virus using 2 commercial hollow-fiber ultrafilters.

• Tác giả: Mark A Borchardt, Tucker R Burch, Rachel M Cook, Gabrielle E Doughan, Aaron D Firnstahl, Joseph A Heffron, Locke A Karriker, Karen M Krueger, Sarah A Opelt, Joel P Stokdyk
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc , 2025
• Bộ sưu tập: NCBI
• ID: 734653

Tóm tắt

 Groundwater near swine farms is an uninvestigated reservoir for porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCVs). Enteric microorganisms are often collected from groundwater via dead-end ultrafiltration, but recovery of PRRSV and PCV with this method has not been assessed. We recovered PRRSV2 and PCV2 by dead-end ultrafiltration followed by polyethylene glycol (PEG) precipitation, nucleic acid extraction, and reverse-transcription quantitative real-time PCR. We also compared 2 commercial hemodialysis ultrafilters (Asahi Kasei Rexeed-25A, Nipro Elisio-25H) and compared PRRSV2 recovery in these filters to other waterborne microorganisms. On average, 8 ± 1% of PRRSV2 was recovered by dead-end ultrafiltration and PEG precipitation, compared to 25 ± 6% for adenovirus 41. Full-process recovery of bacteria in the same filters was 5-15%