Tsampa, which is abundant in polyphenols, demonstrates significant bioactivity and potential health benefit. However, the bioaccessibility and potential bioactivity of polyphenols derived from tsampa prepared from autochthonous lactic acid bacteria solid-fermented roasted highland barley flour (F-RHBF) have not been investigated. This study aimed to evaluate the bioaccessibility and bioactivity of polyphenols from tsampa prepared from F-RHBF through in vitro digestion model, and additionally, to explore the protective effects of digested tsampa extract against oxidative stress damage by establishing H