Integrated microbiome and metabolomics analysis of spoilage characteristics of modified atmosphere packaged pork.

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Tác giả: Kezhou Cai, Xingguang Chen, Wei Cui, Ping Li, Shenyu Ni, Xueya Wang, Baocai Xu, Huaxing Xu, Yujuan Xu, Hui Zhou

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Canada : Food research international (Ottawa, Ont.) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 734701

The interactions between pork microbial communities and metabolites in modified atmosphere packaged (MAP) storage remain unclear. This study exposed the core microbial communities and metabolite profiles during refrigerated pork storage under MAP and illuminated the relationship between them by high-throughput sequencing and non-targeted metabolomics to comprehend the spoilage mechanism induced by microbial activity in MAP pork during storage. The results showed that Pseudomonas and Serratia were the predominant spoilage bacteria in the preliminary stages of refrigerated pork, while Brochothrix gradually dominated in the final stages of storage. 76 differential metabolites were identified from 822 metabolites, consisting of small-molecule metabolites including glycerophospholipids, bitter amino acids, amines, and nucleotides. The metabolic pathways involved in these metabolites were 10 metabolic pathways inclusive of purine metabolism, nucleotide metabolism, and glycerophospholipid metabolism. Correlation results revealed that bacterial genera like Pseudomonas, Brochothrix, Stenotrophomonas, Acinetobacter, and Aeromonas were significantly correlated with metabolites such as lipids, organic acids and nucleotides. These findings enhance our understanding of the spoilage mechanism of refrigerated pork stored in MAP.
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