A nano-sensor based on N-doped carbon dots (NCDs)@ZnO (NCZ) composite was fabricated and efficacy for detecting glucose from human blood and urine samples in a straightforward manner was examined. The composite was prepared following a green hydrothermal method under ambient condition using a novel plant material, Dregea volubilis fruit and structural and optical properties were evaluated using standard techniques. The composite exhibited excellent characteristics including good photostability, biocompatibility, low toxicity, and strong fluorescence, with a decent quantum yield of up to 59%. The NCZ composite has been very sensitive and could selectively detect glucose in urine and blood samples. Selective glucose quenching was efficacious at different concentrations of glucose (1-6 mM) and in the pH range of 7-8, limit of detection was 0.25 mM. The potential uses of carbon-based materials have grown, thanks to the excellent sensing/detection capabilities of the NCZ composite as well as the capacity to prevent nanoparticle aggregation, opening up new possibilities for the development of environmentally benign nano-sensors.