Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection.

• Tác giả: Kurat Ul Ain, Muzamil Bashir, Sanchay Kumar Biswas, Karam Chand, Ankita Chauhan, Divakar Hemadri, Madhusudan Hosamani, Sushmita Nautiyal, Vanitha Selvarajan, Gaurav Kumar Sharma, B P Sreenivasa
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : Journal of immunological methods , 2025
• Bộ sưu tập: NCBI
• ID: 737123

Tóm tắt

 Bluetongue (BT) is a vector-borne viral disease of multiple domestic and wild ruminants across the globe. The VP7 protein of bluetongue virus (BTV) is the major immune-dominant structural protein that is conserved across the BTV serotypes and therefore, targeted for the development of immuno-diagnostics for BT. In this study, full-length recombinant VP7 protein (rVP7) of BTV-1 was expressed in Trochoplusia ni derived insect cells (Tn5) using codon-optimized synthetic gene construct through baculovirus expression system. The seed stock of recombinant baculovirus was amplified to a high titre (>
 1 × 10