Comprehensive quantification of C4 to C26 free fatty acids using a supercritical fluid chromatography-mass spectrometry method in pharmaceutical-grade egg yolk powders intended for total parenteral nutrition use.

• Tác giả: Jonas Bergquist, Anh Vu Nguyen, Mark Dennis Chico Retrato, S J Kumari A Ubhayasekera
• Ngôn ngữ: eng
• Ký hiệu phân loại: 633.15 *Com
• Thông tin xuất bản: Germany : Analytical and bioanalytical chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 737305
• Chủ đề: 1. Comprehensive; 2. Quantification; 3. Of

Tóm tắt

Free fatty acids (FFAs) are important energy sources and significant for energy transport in the body. They also play a crucial role in cellular oxidative stress responses, following cell membrane depolarization, making accurate quantification of FFAs essential. This study presents a novel supercritical fluid chromatography-mass spectrometry (SFC-MS) method using selected ion recording in negative electrospray ionization mode, enabling rapid quantification of 31 FFAs within 6 min without derivatization. FFAs are identified and quantified using an HSS C18 SB column and a secondary mobile phase consisting of methanol with formic acid by detecting their [M - H]