The root ducts play an important role in the plant's transport of nutrients from the soil. Based on the selective transport characteristics of plant roots, amino pillar[6]arene bionic porous root sub-nano channel membrane were constructed to remove Imazamox. Imazamox (IM) is an effective imidazolinone herbicide frequently utilized in soybean fields to control a wide range of annual grasses and broad-leaved weeds. However, it is important to note that while IM is effective in controlling the growth of weeds, its residues can affect the plants that are later planted. Developing a material that can efficiently and selectively remove IM from the environment poses a significant challenge in the realm of agricultural residue removal. A layer-upon-layer covalent assembly was formed through the reaction of amino pillar[6]arene with terephthalaldehyde using an aldehyde-amine Schiff-base reaction. The cavity of amino pillar[6]arenal is used to realize the host-guest interaction of IM. This enabled the development of a membrane with high selectivity for the extraction and removal of IM. The removal rate is 4.66-21.91 times higher than that of other pesticide chemicals. The successful development of the highly selective porous root bionic membrane provides a broad prospect for its application and development in agricultural production.