BACKGROUND: Diseases transmitted by Aedes aegypti or Anopheles stephensi, such as Zika virus, dengue fever, and malaria, pose substantial risks to public health, particularly in tropical areas. Plant-derived compounds have emerged as promising alternatives due to their inherent safety and potential efficiency against mosquitoes. This study aimed to improve the efficacy of certain natural compounds, including α-pinene, citral, camphor, and thymol, by developing nanoliposomal formulations. METHODS: The nanoliposomes containing α-pinene, citral, camphor, and thymol were prepared using the ethanol injection method and then characterized. Using WHO-recommended guidelines, their larvicidal efficacy was investigated against Aedes aegypti and Anopheles stephensi. RESULTS: The nanoliposomes particle sizes were 105 ± 7, 86 ± 5, 149 ± 5, and 135 ± 8 nm, and zeta potentials were - 25.1 ± 0.5, -17.2 ± 1.2, -16.4 ± 1.6, and - 21.3 ± 1.7 mV, respectively. In addition, the ATR-FTIR (Attenuated Total Reflectance-Fourier Transform InfraRed) analysis verified the successful loading of the compound. Nanoliposomal compounds exhibited superior performance compared to their non-formulated counterparts in larvicidal bioassays. The nanoliposomes containing thymol showed the highest efficacy, with a Lethal Concentration 50 (LC CONCLUSIONS: The results suggest that nanoliposomes have the potential to serve as an effective vehicle to improve the efficiency of plant-based larvicides. This could play a significant role in developing sustainable mosquito control strategies.