Light-intersecting Photoclick Reactions for Bioorthogonal Labeling on Single Cells: Dibenzo[b,f][1,4,5]thiadiazepine-11,11-dioxide as a Photoswitchable Reporter.

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Tác giả: Pengchi Deng, Changwei Hu, Fengying Lan, Sitong Li, Zhihao Liu, Zhishan Su, Zhipeng Yu, Cefei Zhang, Xiaohu Zhao, Yuqiao Zhou

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Germany : Angewandte Chemie (International ed. in English) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 738317

The advancement of ring-strain preloaded dipolaro-/dienophiles plays a crucial role in bioorthogonal chemistry, enabling multiple high-precision conjugations toward biomolecules simultaneously. However, durability of these ring-strain preloaded reagents in vivo is a concern, as the ring-strain is not reloadable once released during delivery process. In situ conversion of light-energy into ring-strain is a promising approach to ensure both biostability and spatiotemporal control endowed by light. Herein, we advance a seven-membered cyclic azobenzene photoswitch, dibenzo[b,f][1,4,5]thiadiazepine-11,11-dioxide (DBTDD), bridged by a sulphone moiety. The photoisomerization from Z-DBTDD to ring-strain-loaded E-DBTDD enables an accelerated cycloaddition with various photogenerated dipoles to establish novel photoclick reactions, featuring a dual-λ (405 nm+445 nm) synergistic control. In reactions with monoarylsydnones, a higher photo-stationary ratio of E-DBTDD, achieved by varying the power density of 445 nm laser, presented an ultrafast cycloaddition rate (k
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