The increasing incidence of infertility has increased the cost of assisted reproductive techniques and raised serious concerns on fertilization worldwide. Age and smoking adversely affect the reproductive system. This study aimed to assess the in vitro fertilization (IVF) cycle characteristics and outcomes of smoking women ages over 35 years. The data of 329 women ages 20-35 who underwent frozen embryo transfer in IVF treatment were used in the study. The M2 number, pregnancy outcome, live birth rate, and embryo transfer day showed statistically significant differences according to age group. The 2PN rate, pregnancy outcome, and live birth rate were significantly higher in nonsmoking women with infertility than in smoking women. The effects of these factors, especially smoking behavior, which can potentially be modified to increase the chance of IVF success, on IVF cycle characteristics should be explained to patients.