Protocol for converting assigned nuclear Overhauser enhancement spectroscopy series peaks into exact distance restraints using MATLAB- or CYANA-eNORA.

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Tác giả: Natalie G Ahn, Morkos A Henen, David Jones, David McCall, Beat Vögeli

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : STAR protocols , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 738909

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In the exact nuclear Overhauser enhancement (eNOE) method, tight upper and lower interatomic distance restraints for structure calculations and comparison are extracted from NMR nuclear Overhauser enhancement spectroscopy (NOESY) spectra of biomolecules recorded with different mixing times. Here, we present a protocol for exporting the resonance assignments of NOESY spectra from CCPNmr (v.2) into nmrDraw to obtain peak intensities. We then detail procedures for converting them into distances using either the CYANA- or MATLAB-based "exact NOE by relaxation matrix analysis" (eNORA) program.

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