Glycine betaine (GB) is commonly used as an osmotic regulator and a donor to facilitate changes in methylation in plants and animals, thereby enhancing stress resistance. However, low temperature combined with low light stress represent the most prevalent stresses during pepper growth period in northwest China, and limited studies have focused on the potential stress-mitigating effects of GB. Therefore, to examine the regulatory mechanism of GB-induced tolerance to LL stress, pepper seedlings were pretreated with 20 mmol L