Members of Dehalococcoides mccartyi (Dhc) are strictly anaerobic and play crucial roles in the restoration of many industrial sites impacted by chlorinated solvents, such as tetrachloroethene (PCE) and trichloroethene (TCE). In situ bioremediation with Dhc involves intricate procedures intended to minimize oxygen intrusion, and achieving optimal dechlorination performance in aquifers near the dense non-aqueous phase liquids source zone is challenging. Here, we respectively embedded Dhc strain 195 and the biomass of a Dhc-containing, PCE-dechlorinating consortium in poly(vinyl alcohol)-alginate hydrogel beads. The ethene-forming potential was well-retained in immobilized Dhc following a prolonged oxygen exposure spanning from 12 hours to 7 days, with dechlorination rates ranging from 54.6 ± 4.2-101.9 ± 13.5 µM Cl