The study of antioxidant activity of wild Sambucus Nigra L flower extracts was performed by UV-Vis absorption spectroscopy using two different assays. The analysis of organic acids, fatty acids, ascorbic acid and heavy metals is also performed by virtue of HPLC-DAD, GC-MS and AAS. For these studies, the plant materials were collected from three different regions of Armenia to exhibit the effect of growth place of plant on its composition. Moreover, three different extraction techniques (steam distillation, maceration, Soxhlet extraction) were used to reveal the most effective extraction method for obtaining bioactive compounds from the plant. During extraction ethanol-water solvent was also used to reveal the effect of polarity of the solvent on extraction effectiveness. The obtained results show that both place of growth and extraction method strongly affect the amount of bioactive compounds in the extracts. Moreover, the most effective extraction techniques to obtain various bioactive compounds were revealed.