Dynamic compression modulates anabolic and catabolic activity in chondrocyte seeded agarose constructs.

• Tác giả: Roberto Tarantino, Stephen D Waldman, Peter Zastawny, Lea Zila
• Ngôn ngữ: eng
• Ký hiệu phân loại: 530.429 Liquid crystals
• Thông tin xuất bản: United States : Journal of biomechanics , 2025
• Bộ sưu tập: NCBI
• ID: 739223
• Chủ đề: 1. Dynamic; 2. Compression; 3. Modulates

Tóm tắt

Mechanical stimulation is a widely used technique in the development of tissue engineered cartilage. While various regimes can enhance tissue growth and improve construct mechanical properties, existing outcome measures predominantly assess the anabolic effect of mechanical stimuli. Catabolic responses are generally overlooked, and a critical gap remains in how mechanical loading simultaneously affects both anabolic and catabolic processes. In this study, full-thickness articular cartilage was aseptically harvested from the metacarpal-phalangeal joints of skeletally mature bovine. Isolated chondrocytes were encapsulated in agarose gels and subjected to dynamic compressive strains from 0 % to 15 % for either 20 or 60 min using a custom-built mechanical stimulation device. Anabolism was assessed by [