Loop diuretics mitigate juvenile immobilization treatment-induced hippocampal dysfunction.

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Tác giả: Peng-Kai Chang, Wei-Hsing Lin, Kwok-Tung Lu, Yu-Hsuen Tung, Zong-Syun Wu, Shih-Te Yang, Yi-Ling Yang

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Netherlands : European journal of pharmacology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 739524

Juvenile traumatic experiences can lead to adult cognitive impairments, including learning deficits and increased anxiety risk. Dysfunction of the hippocampus is crucial in stress-induced behavioral disorders, and recent evidence suggests that disrupted chloride homeostasis through the chloride transporter NKCC1 may alter GABAergic signaling and contribute to neuropathology. This study investigates the role of NKCC1 in long-term hippocampal dysfunction induced by juvenile immobilization (J_IMO). Male C57BL/6 mice underwent J_IMO treatment at five weeks of age and were assessed at six and twelve weeks using inhibitory avoidance (IA), open field tests (OFT), extracellular recording, qPCR, and Western blot analyses. Following J_IMO treatment, mice exhibited significant learning deficits in IA, with no notable differences in total movement distance in the OFT. Electrophysiological analysis revealed a marked increase in long-term potentiation (LTP) within the hippocampal Schaffer collateral pathway, while paired-pulse facilitation remained unchanged. An altered input-output curve indicated post-synaptic dysregulation in J_IMO-treated mice. Additionally, Western blot and qPCR analyses showed significant upregulation of Slc12a2 (NKCC1) expression, primarily localized to neural cells, as confirmed by double-staining immunohistochemistry. These findings suggest that NKCC1 plays a pivotal role in J_IMO-induced hippocampal dysfunction, particularly by impairing GABAergic inhibitory neurotransmission. The GABA
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