OBJECTIVES: To compare the biomechanical performance of an all-suture anchor construct to that of both a spiked washer and cancellous screw, and a bone staple in tibial-sided superficial medial collateral ligament (sMCL) reconstruction. METHODS: A time-zero cadaveric biomechanical study was conducted using 24 human cadaveric tibia, with fresh-frozen peroneus longus allografts used for a sMCL reconstruction. Specimens were randomized to one of three treatment groups for tibial fixation being an all-suture anchor construct, spiked washer, or bone staple. A servohydraulic uniaxial testing machine was used to assess elongation after 1,000 cycles at 20-50N, maximum load-to-failure (LTF), elongation at maximum LT, and modes of failure. Differences between groups were analyzed using one-way analysis of variance (ANOVA). RESULTS: No statistically significant differences were observed across the groups. Mean elongation after cyclic loading was 1.1 ±0.7mm in the all-suture anchor group, 0.6 ±1.2mm in the spiked washer group, and 1.1 ±0.6mm in the staple group (p =0.732). Mean maximum LTF was 132.1 ±40.2N for the all-suture anchor group, 120.8 ±54.9N for the spiked washer group, and 123.9 ±64.1N for the staple group (p =0.605). Mean elongation at maximum LTF was 8.6 ±4.1mm for the all-suture anchor group, 8.7 ±5.4mm for the spiked washer group, and 10.8 ±6.3mm for the staple group (p = 0.605). The mode of failure for all specimens was tendon pullout. CONCLUSION: The all-suture anchor construct demonstrated comparable performance to both the spiked washer and bone staple for tibial-sided sMCL reconstruction in this time-zero cadaveric biomechanical assessment.