Molecular analysis of influenza A(H3N2) in a remote tropical island during 2014-2019 to identify the frequency of introduction and local circulation.

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Tác giả: Sikandar Azam, Samantha Louise Bado, Joanne De Jesus Cornejo, Clyde Dapat, Takeaki Imamura, Marianette Inobaya, Christina Dahlia Joboco, Socorro Lupisan, Joanna Ina Manalo, Michiko Okamoto, Hitoshi Oshitani, Beatriz P Quiambao, Mayuko Saito, Mariko Saito-Obata, Yusuke Sayama, Veronica Tallo, Raita Tamaki

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Canada : International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 739565

BACKGROUND: The sources of new antigenic Influenza A(H3N2) variants and the role of tropical regions in the global A(H3N2) circulation remain unclear. By conducting molecular analysis, this study aimed to identify A(H3N2) introduction events and the duration of local circulation in a geographically remote tropical island. METHODS: Nasopharyngeal/nasal samples were collected from symptomatic children under five years old in a remote tropical island of the Philippines between 2014-2019. From the 330 A(H3N2) strains detected, 150 were sequenced for the Hemagglutinin 1 (HA1) gene. Time-scaled Bayesian phylogenetic analysis identified introduction events from global A(H3N2) circulation. We estimated the duration of local circulation and its association with detected amino acid substitutions. RESULTS: Six different A(H3N2) clades/subclades circulated during the study period. Of the 15 introduction events identified during this period, 13 resulted in local circulation lasting less than three months, while one led to five-month-long circulation. Another 10-month-long local circulation event, by definition, was more likely the result of two distinct introduction events with local circulation lasting less than three months, respectively. Most amino acid substitutions that emerged during local circulation were sporadic. No fixed substitutions appeared at the seven key amino acid sites for antigenic changes, suggesting that introduced strains were maintained without the emergence of new antigenic variants. CONCLUSION: In our study population, A(H3N2) circulation resulted from multiple introduction events, followed by local circulation lasting less than three months, with occasional long-term persistence. Our study indicates that tropical regions may contribute to maintaining globally circulating strains but may not be a source of antigenic variants.
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