BACKGROUND: Patellofemoral pain (PFP) affects many women's movement function and quality of life. PFP is related to changes in muscle activity and movement patterns during functional tasks. This study aimed to determine whether the combined analysis of kinematics and electromyography data enhances the ability to discriminate between women with and without PFP, compared with the independent analysis of kinematics and electromyography. METHODS: Thirty-seven women with PFP and 34 unimpaired controls were evaluated for kinematics and electromyography during the lateral step-down (LSD) task. For three-dimensional kinematics, movements in the sagittal, frontal, and transverse planes of the trunk, pelvis, hip, knee, and foot were assessed. For electromyography, filtered, rectified, and smoothed signals from the adductor longus, gluteus medius, vastus lateralis and medialis, rectus femoris, biceps femoris, medial gastrocnemius and tibialis anterior muscles were used. The artificial neural network-based movement deviation profile (MDP) was used to analyse kinematics, electromyography and kinematics combined with electromyography. A MANOVA of MDP RESULTS: Multivariate analysis showed group interaction. There was a significant difference between groups in the Z-score for kinematics. However, no significant differences were observed between groups for electromyography and kinematics combined with electromyography. CONCLUSION: Women with PFP exhibit altered movement patterns during the LSD task but no change in the MDP of muscular activity. Using the MDP, which can combine kinematic and electromyography variables from different segments and muscles, kinematics was the most influential in distinguishing between women with and without PFP.