A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1.

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Tác giả: Yanlong Gu, Jiang Li, Zhipeng Niu, Ya-Fei Song, Ran Wu, Dong-Hui Zhou

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Netherlands : Veterinary parasitology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 740137

Toxoplasmosis is a zoonotic protozoan disease caused by Toxoplasma gondii. Swine serve as intermediate hosts in the transmission of T. gondii, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect T. gondii in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the T. gondii detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The T. gondiii positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against T. gondii, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.
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