Analysts in drug testing laboratories are frequently exposed to drugs as part of their daily work activities. Certain drug components can vaporise and adhere to atmospheric particles. Therefore, the presence of drugs in laboratory settings can affect the health and well-being of the laboratory personnel. This study aimed to highlight possible health consequences of 25 expected drug exposure risks by examining fingernail, hair, and atmospheric environment samples. The results showed that etomidate was detected in the hair samples of eight individuals, with the highest level of 19.73 pg/mg, and no components were detected in the fingernail samples. This indicates that etomidate may adhere to hair but does not significantly affect the human body. An atmospheric particulate sampler was used to collect atmospheric particulate matter and gaseous samples from different laboratory rooms. Methamphetamine, ketamine, etomidate, and other drugs were detected in atmospheric particles and gases. Furthermore, the distribution of these chemicals was evaluated by comparing their concentrations in total suspended particulate matter and gas phase. The development and application of these methods coupled with data analysis, aimed at enhancing the self-protection awareness of laboratory scientists and establish a foundation for future assessments of potential hazards.