Radiotherapy is widely acknowledged as one of the most effective treatments for solid and metastatic tumors. The sensitivity of tissues or cells to radiation is typically estimated using survival curves derived from laboratory experiments with in vitro cell culture models. However, some radioresistant cancer cells can pose significant treatment challenges. High atomic number (Z) nanoparticles, known as radiosensitizing agents, can induce substantial radiosensitization when positioned near therapeutic targets, resulting in a dose increase unattainable by conventional methods. This effect is associated with the emission of secondary electrons by high atomic number materials, effectively transforming them into secondary radiation sources. This study utilized a high-resolution voxelized computational model of an in vitro culture medium to investigate the insertion and impact of gold particles. Monte Carlo code (MCNP6.2) was used to model and simulate a brachytherapy scenario with a High Dose Rate (HDR)