In recent years, the integration of metallic nanoparticles with conductive polymers has gained significant attention for biomedical applications, including biosensors and therapeutic agents. Here, we synthesized hybrid nanocomposites of polyaniline (PANI) with copper (Cu) and silver (Ag) nanoparticles using a straightforward and scalable one-pot synthesis method. These composites were characterized by a range of techniques, including UV-vis, Fourier Transform Infrared (FTIR) spectroscopy, Zeta Potential measurements, Dynamic Light Scattering (DLS), and photoluminescence (PL). Our analysis confirmed that the composites consist of the leucoemeraldine form of PANI, which exhibited dual fluorescence emission at 342 nm (UV region) and 667 nm (visible region), highlighting a synergistic interaction between the metal nanoparticles and PANI chains. The fluorescence quantum yield was determined to be 8.80 % for Cu/PANI and 10.05 % for Ag/PANI, indicating efficient luminescence and tunability based on the metal used. The cytocompatibility was evaluated through MTT assays on four cell lines (Vero cells, macrophages, HeLa cells, and fibroblasts), showing favorable biocompatibility across most cell types. Notably, macrophages (CC