The mechanotransduction (MET) channels of cochlear hair cells is a heteromeric protein complex consisting of TMC1, TMIE and CIB2. The activity of this ion channel is thought to be regulated by a gating spring, a mechanical element that conveys sound-induced vibrations to the MET channel. In nematodes, orthologs of TMC-1, TMIE and CIB2 similarly assemble into a MET channel mediating light nose-touch. Studies in nematodes have suggested that nematode Unc-44, an ortholog of the mammalian ankyrins Ank1, 2, and 3, encodes a gating spring that tethers the nematode MET channel to the cytoskeleton. Here we show that mammalian ankyrins are expressed in cochlear hair cells. Using single and triple conditional knockout mice, we demonstrate that Ank1, 2, and 3 are dispensable for the function of cochlear hair cells. We concluded that Ank1, 2, and 3 are unlikely to be components of the gating spring that gates mechanotransduction channels in mammalian cochlear hair cells.