Synthesis and physicochemical properties of new phosphoramide oligodeoxyribonucleotides. I. N-caffeine derivatives.

• Tác giả: Alexander A Lomzov, Alina I Novgorodtseva, Svetlana V Vasilyeva, Aleksey Y Vorob'ev
• Ngôn ngữ: eng
• Ký hiệu phân loại: 378.242 Thesis and dissertation requirements
• Thông tin xuất bản: United States : Bioorganic chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 740357

Tóm tắt

Herein, we report the synthesis of new oligonucleotide derivatives containing an internucleotide N-caffeine phosphoramide (PN-caffeine) group. This modification was introduced via Staudinger's reaction with an appropriate caffeine azide during standard solid-phase oligonucleotide synthesis. Modified hexathymidylates and oligonucleotides with a heteronucleotide sequence were obtained with a yield of ∼90 %. Physicochemical properties of the new oligonucleotides were analyzed next. UV spectral determination of pK