Red palm weevil (RPW) Rhynchophorus ferrugineus is destructive pests of almost all kind of palms. Acid phosphatase (AcP) distributed in different major tissues and during larval development, the whole gut and the 11th instar larvae displayed the highest activity. Two isoenzymes were detected in the late stages. The purified predominant AcP isoenzyme has molecular weight 30 kDa, optimum activity at pH 5.0 and temperature 40 °C. The enzyme hydrolyzed various phosphoric esters has a Km value 3.33 and 5.0 mM for p-NPP and α-NP as substrates, respectively. It differentially affected by metal cations, different compounds and inhibitors. Ammonium molybdate completely inhibited the enzyme at 50 μM. Tartaric acid, oxalic acid and L-phenyl alanine competitively inhibited the enzyme with Ki values ranged from 0.044 to 0.34 mM. The enzyme differentially inhibited by different botanical extracts, Cinnamomum camphora completely inhibited the enzyme at 65 mg/ml, noncompetitively inhibited by gallic acid with Ki 2.8 mM. It has different susceptibility towards different insecticides, competitively, non- competitively and mixed competitively inhibited with Ki ranged from 0.16 to 1.0 mM. Knowledge the resistance status of RPW is important for researchers to guide the farming community in combating problems and paving the way for developing the management strategies.