BACKGROUND: HIV self-testing may help achieve the UNAIDS 95-95-95 targets because it has the potential to increase testing among equity-denied communities. In 2023, the Public Health Agency of Canada made a one-time million investment into HIV self-testing. We sought to evaluate the outcomes of HIV self-testing, compared to serology in Ontario, Canada. METHODS: We submitted data requests to all agencies involved HIV self-test distribution in Ontario, Canada for 2022-2023. We obtained matching data from the Public Health Ontario Laboratory. We then analyzed for unique test, unique tester, and positivity rate per testing modality. RESULTS: During the analysis period, we found that the laboratory completed an average of 53,606 tests per month for an average number of 44,671 unique persons. For self-tests, there was an average of 1700 tests distributed per month to an average of 678 unique persons. The positivity rate for self-testing was 0.27%, compared to 0.1% for serology. CONCLUSIONS: Our results highlight that self-testing can play a role but will not, alone, achieve the UNAIDS 95-95-95 targets. In our jurisdiction, self-testing corresponded with a higher positivity rate but accounted for only a minority of new diagnoses. In short, HIV self-testing is a tool, but not the solution to the HIV epidemic.