PURPOSE: Research on the effectiveness of food fortification in addressing vitamin D deficiency among Indians is scarce. Thus, this study aimed to assess the effectiveness of consumption of vitamin D fortified foods among families living in Pune, India over 2 years. METHODS: Data in this community-based, longitudinal study were collected from 104 families recruited in two arms [Fortified: 51 families (180 participants)
Unfortified: 53 families (173 participants)]. Liquid Chromatography Tandem Mass Spectrometry was used to estimate serum 25OHD RESULTS: Consumption of vitamin D rich foods was rarely reported. Participants in the fortified arm consumed an average of 42.5% of the RDA for vitamin D (255 IU vitamin D/day) throughout the study period. Endline 25OHD CONCLUSION: The current vitamin D fortification strategy in India, while effective in maintaining the vitamin D TRIAL REGISTRATION: Clinical Trials identifier NCT05541094 (date: 21st March 2023)-retrospectively registered.