BACKGROUND: Developing region-specific dietary strategies is crucial for mitigating methane (CH RESULTS: The results indicate that supplementing the confined feeding ration (FR group) with Sodium Nitrate (12 mmol/L, FRN group), Medicago sativa L. (25%, FRM group), and Helianthus tuberosus L. (3%, FRH group) all have the effect of reducing CH CONCLUSIONS: CH