Biocatalysis is now a well-established branch of catalysis and the growing toolbox of natural, evolved and designer enzymes is enabling chemistry previously deemed inaccessible. However, most enzyme methodologies have been developed for functional group interconversions, such as the conversion of a ketone into an amine or alcohol, and do not result in the generation of significant 3D molecular complexity. The application of enzyme-triggered reaction cascade methodologies has the potential to transform simple substrates into complex sp