Cloning, Expression, Characterization and in silico studies of l-asparaginase from Vibrio sp. (GBPx3).

• Tác giả: Sedigheh Asad, Solat Eslami, Hossein Mahboudi, Sareh Sadat Mousavi Natanzi
• Ngôn ngữ: eng
• Ký hiệu phân loại: 746.445 *Applique
• Thông tin xuất bản: France : Biochimie , 2025
• Bộ sưu tập: NCBI
• ID: 742273

Tóm tắt

l-asparaginase is a critical therapeutic enzyme for treating acute lymphoblastic leukemia (ALL), a common childhood malignancy. In this study, the l-asparaginase coding sequence from halophilic Vibrio sp. (GBPx3) was cloned, expressed in Escherichia coli, and characterized. The enzyme exhibited a molecular weight of 39.2 kDa and demonstrated a K