The emergence of photochromic fibers has created numerous opportunities in the realm of intelligent textiles and functional materials. However, commercially accessible photochromic fibers are predominantly produced from petroleum-based polymers, which contradicts the current emphasis on sustainability and minimizing carbon emissions. In this work, eco-friendly and fast-reversible photochromic bio-based bacterial cellulose (BC) macrofibers that are combined with 2,2,6,6-Tetramethylpiperidine-1-oxy (TEMPO) -oxidized BC (TOBC) nanofibers and spirooxazine-based photochromic microcapsules (PM) through amide reaction via a simple wet spinning strategy, are developed. The findings suggest that the highest breaking strength of the resulting macrofiber is attained at a PM concentration of 0.2 wt.%, reaching 1.51 cN/dtex, which is 14% greater than that of pure TOBC macrofibers produced. Prepared macrofibers with photochromic properties demonstrate fast response times of just 1 s, durability, and reversible color-changing characteristics when stimulated by ultraviolet (UV) light in the 200-400 nm range. As a proof-of-concept, UV-induced color-changing flowers and patterned textiles are demonstrated by the macrofiber integrated with normal yarns. In conclusion, these innovative bio-based polymer fibers can shine new light into the development of a new generation of anti-counterfeiting and fashion textiles.